Pakistan's foreign minister insisted on Sunday Washington and Tehran must uphold a ceasefire agreement, after marathon talks between the two sides to end the war in the Middle East ended without an agreement.

Iran's foreign ministry said no one had held any expectation that talks with the United States could have reached an agreement within one session after the negotiations in Islamabad stalled on Sunday.

Iran and the United States failed to reach an agreement to end the war in the Middle East, U.S. Vice President JD Vance said Sunday after marathon talks in Islamabad, adding that he was leaving after giving Tehran the "final and best offer".

Two U.S. Navy warships transited the Strait of Hormuz to begin clearing Iranian-laid mines, U.S. Central Command said Saturday -- a claim Tehran denied as the Revolutionary Guards threatened to deal "severely" with military vessels crossing the strategic waterway.

U.S. President Donald Trump said prior to the failure of the Islamabad talks that he was not bothered about any outcome of the negotiations, insisting the United States had come out ahead from the war.

American and Iranian officials were holding face-to-face talks in Pakistan on Saturday, the most significant since the 1979 Islamic revolution as the foes try to end a war that plunged the Middle East into violence and rocked the world economy.

U.S. President Donald Trump vowed Friday to have the Strait of Hormuz open "with or without" Iran's cooperation, and said his top priority in peace talks was to ensure Tehran can't have a nuclear weapon.

U.S. Vice President JD Vance arrived in Islamabad on Saturday for talks with Iran that the Pakistani premier hosting the warring sides called a "make or break" effort to permanently halt weeks of fighting in the Middle East.

Iran demanded a truce in Lebanon and the release of its blocked assets on Friday as U.S. Vice President JD Vance warned Tehran not to "play" Washington, casting doubt on planned ceasefire talks in Pakistan.

Iran's new supreme leader Mojtaba Khamenei said in his latest written message the Islamic republic did not want war with the United States and Israel, but would protect its rights as a nation, state television reported Thursday.